px260 (Addgene inc)
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93
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Addgene inc
px260
Px260, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/px260+plasmid/pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro+(Plasmid+%2342229)/pm41803672-487-31-32
Average 93 stars, based on 54 article reviews
Px260, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/px260+plasmid/pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro+(Plasmid+%2342229)/pm41803672-487-31-32
Average 93 stars, based on 54 article reviews
px260 - by Bioz Stars,
2026-09
93/100 stars
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Amplification:Article Title: MGA loss-of-function variants cause premature ovarian insufficiency Article Snippet: .. Cas9 was amplified from the Article Title: MGA loss-of-function variants cause premature ovarian insufficiency Article Snippet: .. Cas9 was amplified from the Article Title: Rapid generation and selection of Cas9-engineering TRP53 R172P mice that do not have off-target effects Article Snippet: .. The Cas9- coding region was PCR amplified with Phusion DNA polymerase from the Plasmid Preparation:Article Title: MGA loss-of-function variants cause premature ovarian insufficiency Article Snippet: .. Cas9 was amplified from the Article Title: MGA loss-of-function variants cause premature ovarian insufficiency Article Snippet: .. Cas9 was amplified from the Article Title: Beta-2 microglobulin knockout K562 cell-based artificial antigen presenting cells for ex vivo expansion of T lymphocytes. Article Snippet: Aim: The human K562 leukemia cell line as a scaffold of artificial antigen presenting cells (aAPCs) for ex vivo lymphocyte expansion does not usually express major histocompatibility complex (MHC) molecules.. However, when stimulated by supernatants from human T lymphocyte cultures, K562 cells upregulate β-2 microglobulin (B2M) and MHC class I expression, which would induce allo-specific T cells.. Methods: We disrupted the B2M locus in K562 cells by CRISPR/Cas9 and achieved MHC class I-negative K562 cells. Article Title: A small-molecule lycorine derivative reveals Na + /K + -ATPase α3 as an anti-obesity target Article Snippet: IC 50 was calculated by Graphpad Prism 7. .. The T7 promoter sequence was fused with the Cas9 coding region which was cloned from Article Title: Mycobacterium tuberculosis inhibits METTL14-mediated m 6 A methylation of Nox2 mRNA and suppresses anti-TB immunity Article Snippet: .. The T7 promoter sequence was fused with the Cas9 coding region which was cloned from Article Title: Rapid generation and selection of Cas9-engineering TRP53 R172P mice that do not have off-target effects Article Snippet: .. The Cas9- coding region was PCR amplified with Phusion DNA polymerase from the Purification:Article Title: MGA loss-of-function variants cause premature ovarian insufficiency Article Snippet: .. Cas9 was amplified from the Article Title: MGA loss-of-function variants cause premature ovarian insufficiency Article Snippet: .. Cas9 was amplified from the DNA Purification:Article Title: MGA loss-of-function variants cause premature ovarian insufficiency Article Snippet: .. Cas9 was amplified from the Article Title: MGA loss-of-function variants cause premature ovarian insufficiency Article Snippet: .. Cas9 was amplified from the CRISPR:Article Title: Beta-2 microglobulin knockout K562 cell-based artificial antigen presenting cells for ex vivo expansion of T lymphocytes. Article Snippet: Aim: The human K562 leukemia cell line as a scaffold of artificial antigen presenting cells (aAPCs) for ex vivo lymphocyte expansion does not usually express major histocompatibility complex (MHC) molecules.. However, when stimulated by supernatants from human T lymphocyte cultures, K562 cells upregulate β-2 microglobulin (B2M) and MHC class I expression, which would induce allo-specific T cells.. Methods: We disrupted the B2M locus in K562 cells by CRISPR/Cas9 and achieved MHC class I-negative K562 cells. Sequencing:Article Title: A small-molecule lycorine derivative reveals Na + /K + -ATPase α3 as an anti-obesity target Article Snippet: IC 50 was calculated by Graphpad Prism 7. .. The T7 promoter sequence was fused with the Cas9 coding region which was cloned from Article Title: Mycobacterium tuberculosis inhibits METTL14-mediated m 6 A methylation of Nox2 mRNA and suppresses anti-TB immunity Article Snippet: .. The T7 promoter sequence was fused with the Cas9 coding region which was cloned from Clone Assay:Article Title: A small-molecule lycorine derivative reveals Na + /K + -ATPase α3 as an anti-obesity target Article Snippet: IC 50 was calculated by Graphpad Prism 7. .. The T7 promoter sequence was fused with the Cas9 coding region which was cloned from Article Title: Mycobacterium tuberculosis inhibits METTL14-mediated m 6 A methylation of Nox2 mRNA and suppresses anti-TB immunity Article Snippet: .. The T7 promoter sequence was fused with the Cas9 coding region which was cloned from other:Article Title: Cytoplasmic PARP1 links the genome instability to the inhibition of antiviral immunity through PARylating cGAS. Article Snippet: Similarly, T7 promoter sequence and the targeting sequence of cGASwere fused to the guide RNA scaffold whichwas cloned from Polymerase Chain Reaction:Article Title: Rapid generation and selection of Cas9-engineering TRP53 R172P mice that do not have off-target effects Article Snippet: .. The Cas9- coding region was PCR amplified with Phusion DNA polymerase from the |